EpiAirway是一种即用型产品, 正常纤毛黏液组织三维模型组成, human-derived tracheal/bronchial epithelial cells also available as a co-culture system with normal human stromal fibroblasts (EpiAirwayFT). 在气液界面(ALI)培养, 上皮气道概括了屏障的体内表型, 黏膜纤毛的反应, infection, 毒性反应和疾病. 有超过100个技术参考资料, EpiAirway is amenable to acute or long-term chronic studies across a wide range of highly predictive in vitro applications.
EpiAirway exhibits human relevant tissue structure and cellular morphology with high uniformity and reproducibility. 其三维结构由有组织的角蛋白5+基底细胞组成, 产生杯状细胞的粘液, 功能性紧密连接和跳动的纤毛. EpiAirwayFT incorporates human fibroblasts in an extracellular stromal matrix ideal for inflammation and fibrosis research.
The EpiAirway 3D human tissue model is routinely utilized for a range of applications including safety & 风险评估，抗病毒研究，炎症 & 纤维化和给药. Simple protocols and the evaluation of early cellular endpoints allow scientists to acquire data in days, not weeks or months.
Virus Infection
应用EpiAirway研究呼吸道病毒附着, replication, 先天免疫反应和抗病毒药物的开发.
Drug Delivery
Utilize existing transdermal permeation equipment or 正规的网博软件有哪些’s simple single insert permeation devices to assess API permeation and flux.
吸入毒物学
Use EpiAirway (available in several formats for physiological exposure systems) to determine relative safety with the EpiAirway IC-75 assay.
炎症和纤维化
Employ EpiAirway’s diverse donor inventory of healthy and diseased donors (Asthma, COPD, Smoker, Goblet Cell Hyperplasia) to elucidate molecular pathways and assess therapeutic efficacy.
Tissue
Kit: 标准的EpiAirway试剂盒(AIR-100)由24个组织组成. (纸巾“包”包含纸巾, 少量培养基, and plasticware; contact 正规的网博软件有哪些 for specific kit contents)
Formats:
- 9mm & 22mm individual inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm
- 24mm, 12mm & 6.5毫米Transwell钻头，孔径为0.4 μm
- 96-well HTS plates – tissue culture substrate is chemically modified with a pore size of 0.4 μm
Culture: 气液界面
Histology: 3-4 cell layers – pseudostratified, mucociliary morphology and tissue structure
Lot numbers: Tissue lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. 所有组织试剂盒在细胞方面都是相同的, medium, handling, culture conditions, etc.
Shipment: 在室温下补充培养基，琼脂糖凝胶
Shipment day: Every Monday
Delivery: 周二上午通过联邦快递优先服务(美国). 美国以外:周二至周四视地点而定
Shelf life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. However, extended storage periods are not recommended unless necessary. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)
实验时长: Tissue cultures can be continued for THREE (3) MONTHS or more with good retention of normal morphology. 纸巾必须每隔一天喂5次.0毫升维护培养基(AIR-100-MM). Cell culture inserts are placed atop washers (EPI-WSHR) or culture stands (MEL-STND) in 6-well plates to allow us of 5.0 ml. See 技术参考编号631.
Cells
Type: 正常人气管/支气管上皮细胞(NHBE);
Genetic make-up: Single donor
Derived from: 健康、不吸烟(可应要求提供备选供体)
Alternatives: 来自哮喘、慢性阻塞性肺病和吸烟者的NHBE
Screened for: HIV，乙型肝炎，丙型肝炎，支原体
Medium
Base medium: Dulbecco 's Modified Eagle 's Medium (DMEM)
生长因子/激素: 表皮生长因子和其他专有因子
Serum: None
Antibiotics: 庆大霉素5µg/ml(为庆大霉素正常水平的10%)
Anti-fungal agent: Amphotericin B 0.25 µg/ml
pH Indicator: Phenol red
Other additives: Proprietary
Alternatives: Phenol red-free, antibiotic-free, anti-fungal-free medium and tissues are available. 代理商至少在发货前3天被移除.
Assay medium: AIR-100-ASY
Maintenance medium: AIR-100-MM
质量控制和无菌
Visual inspection: All tissues are visually inspected and if physical imperfections are noted, 纸巾被拒绝装运
End-use testing: Transepithelial electrical resistance (TEER) of each EpiAirway lot is measured using an EVOM2 epithelial Volthommeter and Endohm electrode chamber (World Precision Instruments). 最低TEER为300欧姆•厘米2 是否需要QC放行.
Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
病原体筛查: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, 微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or 正规的网博软件有哪些 technical service
不合格批件通知: 如果纸巾没有通过我们的质量检验或无菌检验, the customer will be notified and the tissues will be replaced without charge when appropriate. 因为我们的QC和无菌检测是在出货后完成的, notification will be made as soon as possible (Under normal circumstances, TEER故障将在周三下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
